Recently, it has been found that Perfluorinated Alkyl Acids (PFAAs) are toxic substances and potentially harmful to humans and the environment. Used in some industrial applications for many years, these compounds are very persistent and do not easily degrade in the environment. Furthermore, they are known to be carcinogenic and endocrine disruptors in higher animals. Further evidence has shown the potential for bioaccumulation or transmission through food webs in aquatic systems. The purpose of this study is to test the toxicity and bioaccumulation of three Perfluorosulfonic acids (PFSAs) substances which is a subclass of PFAAs on algae using a standard test on the green algae Scenedesmus obliquus. Multiple cultures of S. obliquus was exposed to a 0.01 mg/L PFSAs mixture over time, and sampled for presence and location of PFSA compounds in the biomass through washing and extraction with methanol. Results showed that the concentration of PFOS decreased dramatically by time in solution water, especially compared to PFHxS and PFBS in the mixture. In addition, the level of PFBS was seen to remain constant for 13 days of exposure. At the end of this time, the total PFSAs concentration found on the Scenedesmus obliquus was 0.0248 Î¼g/L of PFOS, and 0.00199 Î¼g/L of PFHxS, with no amounts of PFBS on the algae. For future work, the Half Maximal Effect Concentration (EC50) for PFOS, PFBS and PFHxS on S. obliquus will provide information about the fate of the mixture of the compounds in the water system and algal cell system. The concentration of Chlorophyll-a and Pheophytin will gauge the cellular health, and measures of bioaccumulation will inform about the fate of PFOS and PFHxS. Results from these experiments will provide some basic knowledge about the fate and transport of PFSAs in aquatic systems, and inform further studies on ecological transfer in food webs of these and other PFAAs compounds.